Joachim Mossige

Postdoctoral Fellow - RITMO Centre for Interdisciplinary Studies in Rhythm, Time and Motion (IMV)

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Email joachim.mossige@imv.uio.no

Mobile phone +4792233276

Room RITMO: Red Room / Physics Dept.: v402

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Visiting address 亚博娱乐官网_亚博pt手机客户端登录sveien 3A Harald Schjelderups hus 0373 Oslo / Dept. Physics Sem S?lands vei 24 0371 Oslo

Postal address Postboks 1133 Blindern 0318 Oslo

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Bio

Joachim received his Ph.D. in microfluidics from the University of Oslo and was a postdoc at Stanford and at UCSB to learn about interfacial flows and miscible fluids. He then worked as a teacher of fluid mechanics at the Norwegian University of Life Sciences, and developed "kitchen flows" as an affordable and accessible learning strategy. Back at the University of Oslo, he now works in a multi-disciplinary team to unravel how cells work in concert to form organs. Applications of his research span from water treatment to contact lens development and drug sensitivity testing.

Image may contain: Product, Font, Line, Drinkware, Diagram. — Overview of Joachim's main expertise in experimental fluid dynamics and soft matter physics: **(a)** microfluidics **(b)** miscible flows **(c)** kitchen flows **(d)** interfacial dynamics. Part (a) adapted from Mossige et al., *PRApplied* 2018. Part (b) adapted from Mossige et al., *Phys. Fluids* 2021. Part (c) adapted from Mossige et al., *Rev. Mod. Phys.* 2023. Image courtesy of Sam Dehaeck. Part (d) adapted from Chandran Suja and Mossige et al. *J. Colloid Interface Sci.* 2022.

Utilizing light-sheet microscopy

As postdoc in the UiO:LifeScience ITOM-project, Joachim built his own light-sheet microscope to characterize how early mammalian embryo models (using so-called mouse gastruloids) grow and develop, and how the first organs form. Especially, he is interested in how cells self-organize and orchestrate their motion to form tissue. To explain the underpinning dynamics, he couples his experiments with 3D simulations.

3d rendered image of a 5 day old (cardiovascular) mouse gastruloid. The interior structures are primitive gut cells (endoderm germ layer). MP1 cell line with Sox17 RFP (endoderm) and T/Bra GFP (mesoderm) reporters. Sample provided by Sergei Ponomartcev. Fused, deconvolved and rendered in Huygens SVI.

Optical sectioning through the anterior part of a 5 day old mouse (cardiovascular) gastruloid. The bright regions are primitive gut cells (endoderm germ layer). Made from MP1 cell line with Sox17 RFP (endoderm) and T/Bra GFP (mesoderm) reporters. Sample provided by Sergei Ponomartcev. Fused, deconvolved and rendered in Huygens SVI.

To fully utilize light-sheet imaging, Joachim also studies collective cell migration and how nerve cells make new connections in live zebrafish with members of the Camila Esguerra Group and he studies the cells' own recycling system (autophagy) in Drosophila embryos in collaboration with the Helene Kn?velsrud's group.

3D rendered image showing how cells invade the brain of a zebrafish larvae (6 dpf). The green signal shows the blood vessels, and the red signal shows the cells. To produce this visualization, image stacks from different angles were fused and deconvolved in Huygens SVI and rendered in Imaris. Samples provided by Wietske van der Ent, Camila Esguerra Group - Chemical Neuroscience, UiO.

Schematic overview of our home-built light-sheet microscope.

Collaborators

Dag K. Dysthe (Dept. Physics) oversees the experimental investigation, and Xian Hu helps out with 3D rendering. To explain the dynamics of collective cell migration, Dr. Richard Ho from Luiza Angheluta-Bauer's group conducts complementary simulations. Postdocs Sergei Ponomartcev and Nathalia Smirnova from Hybrid Technology Hub SFF provide stained gastruloid samples. Steven Ray Haakon Wilson at the Chemistry Department contributes with important mass spectrometry measurements. Joachim reports to the center director at RITMO SFF Alexander Refsum Jensenius.

Funding

The ITOM project is funded by UiO:Life Science.

Other interests

Joachim is an avid climber and a hobby jazz musician.