Molecular heterogeneity in multifocal prostate cancer

Background

Prostate cancer is the most common cancer type in Norway with 13 men being diagnosed with the disease every day. The disease is often, but not always, slow growing and there are difficulties in identifying the men with a more aggressive disease. Prostate cancer is also often multifocal with multiple tumours found within the same prostate.

New sequencing technologies enable detection of virtually all mutations present in the genomes of cancers and can be used to understand which genes are most commonly mutated and driving disease progression. Most genomic research on prostate cancer is, however, focusing on one tumour sample per patient, but the multifocal nature of the disease should not be overlooked. Therefore we have collected a large biobank of prostate cancers with multiple cancer samples from each patient making it possible to investigate the genomic changes within each individual tumour. Also, we are continuously collecting blood samples from the same patients, to investigate the presence of tumour specific alterations in a non-invasive manner.

Project

High throughput sequencing data of both DNA and RNA from multifocal prostate cancers generated by researchers at the Radium Hospital is in place. From these datasets a number of interesting genomic and transcriptomic alterations have been identified and the aim of the master project is to further investigate and validate their role in carcinogenesis, their heterogeneity in multifocal prostate cancer and their presence in liquid biopsies. Methods to be used include, but may not be limited to, PCR, real-time PCR, digital-droplet PCR, Rapid Amplification of cDNA Ends (RACE), and Sanger sequencing.

The Research group

As a master student you will be part of the Genome Biology Group at Department of Molecular Oncology located at the Norwegian Radium Hospital. Here a group of biologists and informaticians investigate cancer genomes and transcriptomes by integrating computational and laboratory based approaches to identify and characterize critical genes involved in the cancer development. The group is an associated member of the Centre of Excellence in Cancer Biomedicine and in the K. G. Jebsen Research Centre for Colorectal Cancer. Supervisors will be postdoc Marthe L?vf, group leader Rolf I. Skotheim, and head of department Ragnhild A. Lothe.

Published Mar. 22, 2018 10:28 AM - Last modified Apr. 19, 2018 8:13 AM

Scope (credits)

60