Introduction
Methylation is a common post-translational modification affecting protein functions, for example their localization or interaction with other proteins and molecules, and may influence signaling and other processes in the cell. Proteins are frequently methylated on lysines and arginines, but also on other amino acid residues, including histidines. The list of histidine-methylated human proteins is constantly growing, however the responsible methyltransferases (MTases) remain largely elusive.
Aim
The aim of this Master project is to identify new MTases responsible for histidine methylation events in the human proteome. Based on our previous experience, a candidate approach will be used to test for potential histidine-specific MTases.
Methods
You will have a chance to apply fundamental molecular and cell biology techniques to make great scientific discoveries! Methods regularly used in our lab include: cloning and mutagenesis, genetic manipulation of human cell lines, expression and purification of recombinant proteins, cell imaging and various biochemical approaches to study protein function and their modifications.
Working environment
We are a tight-knit team of scientists who enjoy working hard, while having a lot of fun! We publish in high impact factor journals, such as Nature Communications and Nucleic Acids Research.
Put your talent into action and join our group!
