BIOS9220 – Protein Crystal Spectroscopy
Course content
The course gives and introduction to protein crystal spectroscopy, which is used in combination with protein crystallography to study the structure and function of redox- and metalloproteins. For these types of proteins the structures determined by protein crystallography do not to fully describe the state of these proteins. The essential information that is missing is the oxidation state, spin-state, protonation and in some cases the coordination. This information is provided by the spectroscopic and theoretical methods taught in this course.
Learning outcome
After completing this course we expect your competence to include the following:
- Know the theory for UV-vis and Raman single-crystal protein spectroscopy, and how to use these methods to study redox- and metalloproteins.
- Have knowledge about the theory for electron paramagnetic resonance (EPR) and theoretical methods (density function theory, QM/MM, quantum refinement) for the study of proteins, and how to use these methods.
- Know how X-rays used to solve the crystal structure of proteins can lead to radiation damage of redox- and metallorproteins, and how to use spectroscopy to monitor these effects.
- Know how to combine these methods for studying protein structure, and how to use them in-situ at the synchrotrons.
- Be able to use the above methods in practice.
Admission to the course
PhD candidates from the University of Oslo should apply for classes and register for examinations through?Studentweb.
If a course has limited intake capacity, priority will be given to PhD candidates who follow an individual education plan where this particular course is included. Some national researchers’ schools may have specific rules for ranking applicants for courses with limited intake capacity.
PhD candidates who have been admitted to another higher education institution must?apply for a position as a visiting student?within a given deadline.
Recommended previous knowledge
Basic knowledge of protein structure and protein structure determination.
Teaching
The course comprises for each student of 21 lecture hours and 16 hours of lab-exercises. The course is taught intensively for one week in the beginning of the autumn term every second year.
A selected set of articles should be studied before course start. Estimated time for this activity: one week.
All teaching sessions are obligatory and constitute the curriculum together with hand-outs and pre-defined articles
Examination
Final written exam which counts 100 % towards the final?grade.?
This course has mandatory exercises that must be approved before you can sit the final?exam.
It will also be counted as one of the three attempts to sit the exam for this course, if you sit the exam for one of the following courses: MBV9220 – Protein Crystal Spectroscopy (continued)
Examination support material
No examination support material is allowed.
Grading scale
Grades are awarded on a pass/fail scale. Read more about the grading system.
Resit an examination
This course offers both postponed and resit of examination. Read more:
More about examinations at UiO
- Use of sources and citations
- Special exam arrangements due to individual needs
- Withdrawal from an exam
- Illness at exams / postponed exams
- Explanation of grades and appeals
- Resitting an exam
- Cheating/attempted cheating
You will find further guides and resources at the web page on examinations at UiO.